期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 32, 期 7, 页码 1277-1309出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2011.196
关键词
blood flow; cranial window; neurovascular coupling; stroke; two-photon microscopy; vasculature
资金
- NIA NIH HHS [F32 AG031620, AG031620] Funding Source: Medline
- NIBIB NIH HHS [R01 EB003832, EB003832] Funding Source: Medline
- NIDA NIH HHS [R01 DA029706] Funding Source: Medline
- NIH HHS [DP1 OD006831, OD006831] Funding Source: Medline
- NIMH NIH HHS [R01 MH085499, MH085499] Funding Source: Medline
- NINDS NIH HHS [DP1 NS082097] Funding Source: Medline
The cerebral vascular system services the constant demand for energy during neuronal activity in the brain. Attempts to delineate the logic of neurovascular coupling have been greatly aided by the advent of two-photon laser scanning microscopy to image both blood flow and the activity of individual cells below the surface of the brain. Here we provide a technical guide to imaging cerebral blood flow in rodents. We describe in detail the surgical procedures required to generate cranial windows for optical access to the cortex of both rats and mice and the use of two-photon microscopy to accurately measure blood flow in individual cortical vessels concurrent with local cellular activity. We further provide examples on how these techniques can be applied to the study of local blood flow regulation and vascular pathologies such as small-scale stroke. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 1277-1309; doi: 10.1038/jcbfm.2011.196; published online 1 February 2012
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据