期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 32, 期 9, 页码 1778-1787出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2012.82
关键词
blood-brain glucose transport; blood-brain barrier (BBB); brain glucose metabolism; cerebral metabolic rate of glucose (CMRglc); in-vivo H-1 MRS; Michaelis-Menten model
资金
- NIH [R21MH092704, NS041262, NS057560, NS070839, P41 RR08079, P41 EB015894, P30 NS057091]
- WM Keck Foundation
Cerebral glucose consumption and glucose transport across the blood-brain barrier are crucial to brain function since glucose is the major energy fuel for supporting intense electrophysiological activity associated with neuronal firing and signaling. Therefore, the development of noninvasive methods to measure the cerebral metabolic rate of glucose (CMRglc) and glucose transport constants (K-T: half-saturation constant; T-max: maximum transport rate) are of importance for understanding glucose transport mechanism and neuroenergetics under various physiological and pathological conditions. In this study, a novel approach able to simultaneously measure CMRglc, K-T, and T-max via monitoring the dynamic glucose concentration changes in the brain tissue using in-vivo H-1 magnetic resonance spectroscopy (MRS) and in plasma after a brief glucose infusion was proposed and tested using an animal model. The values of CMRglc, T-max, and K-T were determined to be 0.44 +/- 0.17 mu mol/g per minute, 1.35 +/- 0.47 mu mol/g per minute, and 13.4 +/- 6.8 mmol/L in the rat brain anesthetized with 2% isoflurane. The Monte-Carlo simulations suggest that the measurements of CMRglc and T-max are more reliable than that of K-T. The overall results indicate that the new approach is robust and reliable for in-vivo measurements of both brain glucose metabolic rate and transport constants, and has potential for human application. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 1778-1787; doi:10.1038/jcbfm.2012.82; published online 20 June 2012
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