期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 32, 期 9, 页码 1788-1799出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2012.85
关键词
cell culture; energy metabolism; free radicals; MR spectroscopy; neurotransmitters
资金
- Danish Medical Research Council [09-063393, 10-094362]
The brain is highly susceptible to oxidative injury, and the pentose phosphate pathway (PPP) has been shown to be affected by pathological conditions, such as Alzheimer's disease and traumatic brain injury. While this pathway has been investigated in the intact brain and in astrocytes, little is known about the PPP in neurons. The activity of the PPP was quantified in cultured cerebral cortical and cerebellar neurons after incubation in the presence of [2-C-13] glucose or [3-C-13] glucose. The activity of the PPP was several fold lower than glycolysis in both types of neurons. While metabolism of C-13-labeled glucose via the PPP does not appear to contribute to the production of releasable lactate, it contributes to labeling of tricarboxylic acid (TCA) cycle intermediates and related amino acids. Based on glutamate isotopomers, it was calculated that PPP activity accounts for similar to 6% of glucose metabolism in cortical neurons and similar to 4% in cerebellar neurons. This is the first demonstration that pyruvate generated from glucose via the PPP contributes to the synthesis of acetyl CoA for oxidation in the TCA cycle. Moreover, the fact that C-13 labeling from glucose is incorporated into glutamate proves that both the oxidative and the nonoxidative stages of the PPP are active in neurons. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 1788-1799; doi:10.1038/jcbfm.2012.85; published online 20 June 2012
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据