期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 31, 期 12, 页码 2267-2281出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2011.104
关键词
astrocyte basal lamina; blood-brain barrier; endothelial cell; proteomics; vascular permeability
资金
- Center for Aids Research
- American Brain Tumor Association
- NINDS [1R21NSO058776]
- NHLBI [R01HL073396]
- CFAR [3 P30 AI036214-13S1]
- [NIH/NS058776]
- [NIH/HL073396]
- [NIH/AI036214]
The blood-brain barrier (BBB) is a multicellular vascular structure separating blood from the brain parenchyma that is composed of endothelial cells with tight intercellular junctions, surrounded by a basal lamina, astrocytes, and pericytes. Previous studies have generated detailed databases of the microvessel transcriptome; however, less information is available on the BBB at the protein level. In this study, we specifically focused on characterization of the membrane fraction of cells within the BBB to generate a more complete understanding of membrane transporters, tight junction proteins, and associated extracellular matrix proteins that are functional hallmarks of the BBB. We used Multidimensional Protein Identification Technology to identify a total of 1,143 proteins in mouse brain microvessels, of which 53% were determined to be membrane associated. Analyses of specific classes of BBB-associated proteins in the context of recent transcriptome reports provide a unique database to assess the relative contribution of genes at the level of both RNA and protein in the maintenance of normal BBB integrity. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 2267-2281; doi:10.1038/jcbfm.2011.104; published online 27 July 2011
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据