期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 30, 期 3, 页码 653-662出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2009.238
关键词
administration route; lesion volume; MRI; neural progenitor cell; rat; transient ischemia
资金
- National Institute of Neurological Diseases and Stroke [P50 NS23393, PO1 NS42345, RO1 NS48349, RO1 NS38292, RO1 NS43324, HL64766]
- Mort and Brigitte Harris Foundation
We tested the hypotheses that administration routes affect the migration and distribution of grafted neural progenitor cells (NPCs) in the ischemic brain and that the ischemic lesion plays a role in mediating the grafting process. Male Wistar rats (n = 41) were subjected to 2-h middle cerebral artery occlusion (MCAo), followed 1 day later by administration of magnetically labeled NPCs. Rats with MCAo were assigned to one of three treatment groups targeted for cell transplantation intra-arterially (IA), intracisternally (IC), or intravenously (IV). MRI measurements consisting of T2-weighted imaging and three-dimensional (3D) gradient echo imaging were performed 24 h after MCAo, 4 h after cell injection, and once a day for 4 days. Prussian blue staining was used to identify the labeled cells, 3D MRI to detect cell migration and distribution, and T2 map to assess lesion volumes. Intra-arterial (IA) administration showed significantly increased migration, a far more diffuse distribution pattern, and a larger number of transplanted NPCs in the target brain than IC or IV administration. However, high mortality with IA delivery (IA: 41%; IC: 17%; IV: 8%) poses a serious concern for using this route of administration. Animals with smaller lesions at the time of transplantation have fewer grafted cells in the parenchyma. Journal of Cerebral Blood Flow & Metabolism (2010) 30, 653-662; doi: 10.1038/jcbfm.2009.238; published online 4 November 2009
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