期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 29, 期 12, 页码 1914-1923出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2009.104
关键词
adsorptive-mediated mechanism; brain-targeting gene delivery; Lf-modified nanoparticles; receptor-mediated mechanism
资金
- National Basic Research Program of China (973 Program [2007CB935802]
- Shanghai Nanotechnology Project [0852nm04500]
Ligand-mediated brain-targeting drug delivery is one of the focuses at present. Elucidation of exact targeting mechanisms serves to efficiently design these drug delivery systems. In our previous studies, lactoferrin (Lf) was successfully exploited as a brain-targeting ligand to modify cationic dendrimer-based nanoparticles (NPs). The mechanisms of Lf-modified NPs to the brain were systematically investigated in this study for the first time. The uptake of Lf-modified vectors and NPs by brain capillary endothelial cells (BCECs) was related to clathrin-dependent endocytosis, caveolae-mediated endocytosis, and macropinocytosis. The intracellular trafficking results showed that Lf-modified NPs could rapidly enter the acidic endolysosomal compartments within 5 mins and then partly escape within 30 mins. Both Lf-modified vectors and NPs showed higher blood-brain barrier-crossing efficiency than unmodified counterparts. All the results suggest that both receptor- and adsorptive-mediated mechanisms contribute to the cellular uptake of Lf-modified vectors and NPs. Enhanced brain-targeting delivery could be achieved through the synergistic effect of the macromolecular polymers and the ligand. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1914-1923; doi:10.1038/jcbfm.2009.104; published online 5 August 2009
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