Investigations into the thiamine and riboflavin content of malt and the effects of malting and roasting on their final content

A simple and effective method for the analysis of thiamine (B-1) and riboflavin (B-2) vitamers, in unmalted and malted grains, by high performance liquid chromatography (HPLC) has been developed. This method makes use of trichloroacetic acid (TCA) as an extraction medium to effectively clean up the sample and analyze the vitamer content with high accuracy (R-2 > 0.9992; %RSD < 5%). This method was employed in the routine analysis of a wide range of malted and unmalted grains, and it was found that lighter colored malts contain higher concentrations (2-5 times higher) of thiamine and riboflavin vitamers compared to darker colored malts. The malting process has no effect on the overall riboflavin content; however, both steeping and kilning processes cause increases in thiamine vitamer content. Roasting profiles show that thiamine and riboflavin vitamer concentrations are significantly effected when the temperature reaches 120 degrees C, which explains why roasted products have lower vitamer content than paler malts. (C) 2012 Elsevier Ltd. All rights reserved.