Identification of buckwheat phenolic compounds by reverse phase high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS)

This study describes the characterization of phenolic compounds in buckwheat. Reverse phase high performance liquid chromatography (RP-HPLC) coupled to electrospray ionization-time of flight-mass spectrometry (ESI-TOF-MS) has been applied to separate and characterize thirty phenolic compounds in buckwheat flour. As far as we know, 2-hydroxy-3-O-beta-D-glucopyranosil-benzoic acid, 1-O-caffeoy1-6-O-alpha-rhamnopyranosyl-beta-glycopyranoside and epicatechin-3-(3 ''-O-methyl) gallate were tentatively identified in buckwheat for the first time. The sensitivity, mass accuracy and true isotopic pattern of the TOF-MS, legitimated the identification of phenolic compounds present in buckwheat extract. Furthermore, other unknown compounds were also reported in the ethanolic extracts of buckwheat (C) 2010 Elsevier Ltd. All rights reserved.