期刊
JOURNAL OF CEREAL SCIENCE
卷 49, 期 2, 页码 254-261出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2008.10.011
关键词
Glu-3; Glu-D3; Low-molecular weight glutenin; Protein; Wheat
资金
- South Australian Premier's Science and Research Fund
- Research Fund
- Molecular Plant Breeding Cooperative Research Centre.
Low-molecular weight glutenin subunits (LWM-GS) are important components of wheat (Triticum aestivum L) gluten, with important effects on end-use quality. The LMW-GS are encoded at Glu-3 loci (Glu-A3, Glu-B3 and Glu-D3. on the short arms of chromosomes 1A, 113 and 113), each of which exhibits extensive allelic variation. Each locus encodes numerous LMW-GS, some of which have similar electrophoretic mobilities, making it difficult to distinguish among Glu-3 loci. Alleles of the Glu-D3 locus of bread wheat are considered the most problematic to assign. To date, six Glu-D3 alleles, designated a, b, c, d, e and f have been reported. We report five previously undescribed alleles (g, h, i, j and k), and describe a method for characterizing them using a combination of SDS-PAGE and multiplexed PCR-based DNA markers. This method could be used for accurate identification of Glu-D3 alleles, permitting the estimation of the effects of these alleles on end-use quality and the selection of desirable alleles and allelic combinations in wheat breeding. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.
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