期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 227, 期 9, 页码 3216-3224出版社
WILEY
DOI: 10.1002/jcp.24012
关键词
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资金
- National Basic Research Program of China [2007CB947304, 2010CB944801]
- Jurisdiction of Beijing Municipality [PHR20090510, PXM 2009-014226-074691, PXM2011-014226-07-000066]
- National Institute of Dental and Craniofacial Research, the National Institutes of Health, the Department of Health and Human Services [R01 DE019932]
- California Institute for Regenerative Medicine [RN1-00572]
- Beijing Municipality
- National Institute of Dental and Craniofacial Research, the National Institutes of Health
Cell sheet engineering has been developed as an alternative approach to improve mesenchymal stem cell-mediated tissue regeneration. In this study, we found that vitamin C (Vc) was capable of inducing telomerase activity in periodontal ligament stem cells (PDLSCs), leading to the up-regulated expression of extracellular matrix type I collagen, fibronectin, and integrin beta 1, stem cell markers Oct4, Sox2, and Nanog as well as osteogenic markers RUNX2, ALP, OCN. Under Vc treatment, PDLSCs can form cell sheet structures because of increased cell matrix production. Interestingly, PDLSC sheets demonstrated a significant improvement in tissue regeneration compared with untreated control dissociated PDLSCs and offered an effective treatment for periodontal defects in a swine model. In addition, bone marrow mesenchymal stem cell sheets and umbilical cord mesenchymal stem cell sheets were also well constructed using this method. The development of Vc-mediated mesenchymal stem cell sheets may provide an easy and practical approach for cell-based tissue regeneration. J. Cell. Physiol. 227: 32163224, 2012. (C)2011 Wiley Periodicals, Inc.
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