期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 115, 期 5, 页码 847-853出版社
WILEY
DOI: 10.1002/jcb.24726
关键词
miR-590-5p; CHL1; CERVICAL CANCER; CELL INVASION; CELL CYCLE
资金
- Science and Technology Planning Project of Sichuan Province, China [2011FZ0041]
MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we identified that miR-590-5p was up-regulated in human cervical cancer. Over-expression of miR-590-5p promoted cervical cancer cell growth, cell cycle and invasion via Growth curve, Colony formation, FACS and Transwell assays in HeLa and C33A cell lines. Subsequently, CHL1 was identified as a potential miR-590-5p target by bioinformatics analysis. Moreover, we showed that CHL1 was negatively regulated by miR-590-5p at the posttranscriptional level, via a specific target site within the 3UTR by luciferase reporter assay. Furthermore, the mRNA and protein levels of CHL1 in cervical cancer cells were downregulated by miR-590-5p. And we identified the cell phenotype altered by miR-590-5p can be rescued by over-expression of CHL1. Therefore, our findings suggest that miR-590-5p acts as an oncogene by targeting the CHL1 gene and promotes cervical cancer proliferation. The findings of this study contribute to current understanding of the functions of miR-590-5p in cervical cancer. J. Cell. Biochem. 115: 847-853, 2014. (c) 2013 Wiley Periodicals, Inc.
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