期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 115, 期 2, 页码 209-216出版社
WILEY
DOI: 10.1002/jcb.24658
关键词
CORRELATED; IMAGING; FLUORESCENCE; MICROSCOPY; SOFT X-RAY; TOMOGRAPHY
资金
- NIH-NIGMS [P41 GM103445]
- US DOE-BER [DE-AC02-05CH11231]
- NIH-NIDDK [Intramural]
Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopes and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT). This amalgamation of techniques integrates 3D molecular localization data (CFT) with a high-resolution, 3D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near-native, cryopreserved state. Here we describe the current state of the art in correlative CFT-SXT, and discuss the future outlook for this method. J. Cell. Biochem. 115: 209-216, 2014. (c) 2013 Wiley Periodicals, Inc.
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