期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 115, 期 2, 页码 232-242出版社
WILEY
DOI: 10.1002/jcb.24655
关键词
miR-34c; NANOS2; SPERMATOGONIAL STEM CELLS (SSCs); DIFFERENTIATION
资金
- National Natural Science Foundation of China [31272518]
- Ministry of Science and Technology of China [2013CB947902]
- Ministry of Education of China [RFDP, 20120204110030, NCET-09-0654]
- Fundamental Research Funds for the Central Universities [QN2011012]
- National Natural Science Foundation of China [31272518]
- Ministry of Science and Technology of China [2013CB947902]
- Ministry of Education of China [RFDP, 20120204110030, NCET-09-0654]
- Fundamental Research Funds for the Central Universities [QN2011012]
miRNAs are expressed in many mammalian cells, acting specific roles in regulating gene expression or mediating special mRNAs cleavage by targeting their 3-untranslated region (3UTR). Some miRNAs are essential and important for animal development. However, it is still unclear what the relationship is between miR-34c and mammalian spermatogonial stem cells (SSCs). We found that a conserved microRNA-34c through its target-Nanos2, regulating SSCs' differentiation in mouse. Immunohistochemistry analysis of Nanos2 and miR-34c FISH results revealed the opposite expression trends between them. Seven bioinformatics websites and programs predicted that miR-34c has interaction sites in Nanos2's 3UTR. Dual-luciferase reporter vector and mutated dual-luciferase reporter vector analysis validated that they are interacted. After transfection miR-34c mimics into mouse SSCs, or miR-34c lentiviral vector in vitro co-cultivation with seminiferous tubules, and Western blot analysis demonstrated that miR-34c over-expression could suppress Nanos2 expression in post-transcription level. Our experiments identified that miR-34c may promote meiosis process by interacting with Nanos2 leading up-regulation of Stra8 in mouse spermatogonial stem cells. J. Cell. Biochem. 115: 232-242, 2014. (c) 2013 Wiley Periodicals, Inc.
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