期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 112, 期 3, 页码 782-792出版社
WILEY-BLACKWELL
DOI: 10.1002/jcb.22954
关键词
EPITHELIAL-MESENCHYMAL TRANSITION/PROTEASOME/TETRASPAN/TRANSCRIPTION
资金
- Basic Research Promotion Fund [KOSEF 2009-0440]
- MOEHRD [KRF-2007-C00186]
- Research Program for New Drug Target Discovery [2007-03536]
- Cell Dynamics Research Center [R11-2007-007-01004-0]
- Ministry of Education, Science and Technology [2010-00150029]
- National Research Foundation of Korea [R11-2007-007-01004-0, 2007-03536] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Transmembrane 4 L six family member 5 (TM4SF5) is highly expressed in hepatocarcinoma and causes epithelial-mesenchymal transition (EMT) of hepatocytes. We found that TM4SF5-expressing cells showed lower mRNA levels but maintained normal protein levels in certain gene cases, indicating that TM4SF5 mediates stabilization of proteins. In this study, we explored whether regulation of proteasome activity and TM4SF5 expression led to EMT. We observed that TM4SF5 expression caused inhibition of proteasome activity and proteasome subunit expression, causing morphological changes and loss of cell-cell contacts. shRNA against TM4SF5 recovered proteasome expression, with leading to blockade of proteasome inactivation and EMT. Altogether, TM4SF5 expression appeared to cause loss of cell-cell adhesions via proteasome suppression and thereby proteasome inhibition, leading to repression of cell-cell adhesion molecules, such as E-cadherin. J. Cell. Biochem. 112: 782-792, 2011. (C) 2010 Wiley-Liss, Inc.
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