4.6 Article

Proteasome Inhibition Causes Epithelial-Mesenchymal Transition upon TM4SF5 Expression

期刊

JOURNAL OF CELLULAR BIOCHEMISTRY
卷 112, 期 3, 页码 782-792

出版社

WILEY-BLACKWELL
DOI: 10.1002/jcb.22954

关键词

EPITHELIAL-MESENCHYMAL TRANSITION/PROTEASOME/TETRASPAN/TRANSCRIPTION

资金

  1. Basic Research Promotion Fund [KOSEF 2009-0440]
  2. MOEHRD [KRF-2007-C00186]
  3. Research Program for New Drug Target Discovery [2007-03536]
  4. Cell Dynamics Research Center [R11-2007-007-01004-0]
  5. Ministry of Education, Science and Technology [2010-00150029]
  6. National Research Foundation of Korea [R11-2007-007-01004-0, 2007-03536] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

向作者/读者索取更多资源

Transmembrane 4 L six family member 5 (TM4SF5) is highly expressed in hepatocarcinoma and causes epithelial-mesenchymal transition (EMT) of hepatocytes. We found that TM4SF5-expressing cells showed lower mRNA levels but maintained normal protein levels in certain gene cases, indicating that TM4SF5 mediates stabilization of proteins. In this study, we explored whether regulation of proteasome activity and TM4SF5 expression led to EMT. We observed that TM4SF5 expression caused inhibition of proteasome activity and proteasome subunit expression, causing morphological changes and loss of cell-cell contacts. shRNA against TM4SF5 recovered proteasome expression, with leading to blockade of proteasome inactivation and EMT. Altogether, TM4SF5 expression appeared to cause loss of cell-cell adhesions via proteasome suppression and thereby proteasome inhibition, leading to repression of cell-cell adhesion molecules, such as E-cadherin. J. Cell. Biochem. 112: 782-792, 2011. (C) 2010 Wiley-Liss, Inc.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据