期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 110, 期 6, 页码 1481-1494出版社
WILEY
DOI: 10.1002/jcb.22715
关键词
L-TYPE Ca (2+) CHANNEL; ERK; Elk-1; p38 PROTEIN KINASE; MKK4; MKK6; c-Jun
资金
- University of Saarland
The transcriptional activity of AP-1 has been analyzed in glucose-stimulated INS-1 insulinoma cells using a chromosomally embedded AP-1-responsive reporter gene. We show that AP-1 activity was significantly elevated in glucose-treated INS-1 cells. Preincubation of the cells with nifedipine or expression of the Ca2+ binding protein parvalbumin in the cytoplasm of INS-1 cells reduced AP-1 activity. Thus, activation of L-type Ca2+ channels and an elevated cytoplasmic Ca2+ concentration are crucial to connecting glucose stimulation with enhanced AP-1 activity. Expression of dominant negative forms of A-Raf, MKK4 or MKK6 and pharmacological inhibition of MEK and p38 revealed that extracellular signal-regulated protein kinase, p38 and c-Jun NH2-terminal protein kinase participate in the upregulation of AP-1 activity. Expression of dominant-negative mutants of c-Jun and Elk-1 reduced AP-1 transcriptional activity in INS-1 cells indicating that c-Jun and ternary complex factors are involved in the regulation of AP-1 activity in glucose-stimulated insulinoma cells. J. Cell. Biochem. 110: 1481-1494, 2010. (C) 2010 Wiley-Liss, Inc.
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