期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 107, 期 4, 页码 741-747出版社
WILEY
DOI: 10.1002/jcb.22169
关键词
RESVERATROL; SIRTs; OXIDATIVE STRESS; CARDIOMYOCYTE APOPTOSIS; CASPASE-3
资金
- Natural Science Foundation of Guangdong Province, China [5008363]
Resveratrol, a polyphenol found in fruits, has been demonstrated to activate Sir2. Though many studies have demonstrated that resveratrol can activate SIRT1, whether it has effect on other sirtuins (SIRT2-7) are unknown. The present study shows that exposure of H9c2 cells to 50 mu M H2O2 for 6 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry (FCM), but pretreatment of resveratrol (20 mu M) eliminated H2O2-induced apoptosis. Resveratrol also prevented H2O2-induced caspase-3 activation. Exposure of cells to resveratrol caused rapid activation of SIRT1,3,4,7. Sirtuin inhibitor, nicotinamide (20 mM) attenuated resveratrol's inhibitory effect on cell apoptosis and caspase-3 activity. These results suggest that resveratrol protects cardiomyocytes from H2O2-induced apoptosis by activating SIRT1,3,4,7. J. Cell. Biochem. 107: 741-747, 2009. (C) 2009 Wiley-Liss, Inc.
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