期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 106, 期 3, 页码 409-413出版社
WILEY
DOI: 10.1002/jcb.22026
关键词
KCTD10; PROLIFERATING CELL NUCLEAR ANTIGEN; CELL PROLIFERATION
资金
- National Natural Science Foundation of China [30740014]
- National 973 Basic Research Project [2006CB503805]
A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel terramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library. Computational analysis showed that KCTD10 cDNA is 2,638 bp long, encoding 313 amino acids with a proliferating cell nuclear antigen binding motif, mapped to chromosome 12q24.11 with 7 exons, ubiquitously expressed in all 12 tested normal tissues and 7 of 8 tested tumor cell lines from MTN membranes by Northern blot. Nuclear localization of KCTD10 was observed in A549 cells. Yeast two-hybrid analysis and immunoprecipitation assay showed that KCTD10 can interact with PCNA. In A549 cells, KCTD10 down-regulation could inhibit cell proliferation, but its over-expression could not influence cell proliferation. The results suggest that KCTD10 may be associated with DNA synthesis and cell proliferation. J. Cell. Biochem. 106: 409-413, 2009. (C) 2009 Wiley-Liss, Inc.
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