期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 106, 期 2, 页码 344-352出版社
WILEY
DOI: 10.1002/jcb.22011
关键词
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; CYTOSKELETON; CAVEOLAE: CA(2+) WAVES; ENDOTHELIUM
资金
- Canadian Institutes of Health Research
- Heart and Stroke Foundation of Quebec
Ca2+ is a highly versatile second messenger that plays a key role in the regulation of numerous cell processes. One-way cells ensure the specificity and reliability of Ca2+ signals is by organizing them spatially in the form of waves that propagate throughout the cell or within a specific subcellular region. In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is responsible for the release of Ca2+ from the endoplasmic reticulum. The spatial aspect of the Ca2+ signal depends on the organization of various elements of the Ca2+ signaling toolkit and varies from tissue to tissue. Ca2+ is implicated in many of endothelium functions that thus depend on the versatility of Ca2+ signaling. In the present study, we showed that the disruption of caveolae microdomains in bovine aortic endothelial cells (BAEC) with methyl-3-cyclodextrin was not sufficient to disorganize the propagation of Ca2+ waves when the cells were stimulated with ATP or bradykinin. However, disorganizing microfilaments with latrunculin B and microtubules with colchicine both prevented the formation of Ca2+ waves. These results suggest that the organization of the Ca2+ waves mediated by IP3R channels does not depend on the integrity of caveolae in BAEC, but that microtubule and microfilament cytoskeleton assembly is crucial. J. Cell. Biochem. 106: 344-352, 2009. (C) 2008 Wiley-Liss, Inc.
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