期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 105, 期 1, 页码 121-128出版社
WILEY
DOI: 10.1002/jcb.21799
关键词
Cas-1; fMLP; Hef1; lipopolysaccharide; Nedd9; neutrophil; tumor necrosis factor alpha
资金
- Japan Society for Promotion of Science [18591079]
- The 21st Century
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Frontier Research on Molecular Destruction and Reconstitution of Tooth and Bone
- Osaka City University Research Foundation
- Grants-in-Aid for Scientific Research [18591079] Funding Source: KAKEN
Crk-associated substrate lymphocyte type (Cas-L) protein, also known as human enhancer of filamentation 1 (Hef1) or neural precursor cell-expressed, developmentally down-regulated gene 9 (Nedd9), belongs to the Cas family of adapter proteins, which are involve(] in integrin signaling. Previous reports showed that Cas-L is expressed preferentially in lymphocytes and epithelial cells. Cas-L mediates signals from integrins, T-cell receptors, B-cells receptors, and transforming growth factor beta, leading to cell movement and cell division. Here, we report the expression of Cas-L in neutrophils. Cas-L was tyrosine-phosphorylated when human neutrophils were stimulated by fMLP, tumor necrosis factor-alpha (TNF), or lipopolysaccharide. The tyrosine phosphorylation of Cas-L in fMLP- or TNF-stimulated neutrophils was further enhanced by adhesion of the cells to their substrates. Cas-L was found to be localized at focal adhesions in stimulated neutrophils based on immunofluorescence microscopy. These findings suggest that Cas-L is one of the targets of inflammatory cytokines and is also modulated by cell adhesion process in neutrophils.
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