期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 22, 期 10, 页码 5020-5028出版社
WILEY
DOI: 10.1111/jcmm.13784
关键词
beta-catenin; gastric cancer; invasion; proliferation; TRIM32
资金
- Shanghai Municipal Health and Family Planning Commission scientific research project [20174Y0222]
- Science and Technology Support Project of Medical Guidance (Chinese and Western Medicine) of Shanghai [17411972200]
The tripartite motif (TRIM) family comprises more than 70 members involved in the regulation of many cellular pathways. TRIM32 acts as an E3 ubiquitin ligase and has been reported to participate in many human cancers. Here, we aimed to investigate the role of TRIM32 in gastric cancer (GC) and the clinical implications. High expression of TRIM32 was observed in GC tissues and cell lines, and was significantly associated with poor prognosis. Knockdown TRIM32 expression remarkably suppressed the proliferation, migration, and invasion of GC cells invitro and tumour growth invivo, whereas overexpression of TRIM32 yielded the opposite results. Western blotting and quantitative reverse-transcription PCR (qRT-PCR) analyses revealed that up-regulation of TRIM32 significantly enhanced expression of beta-cateninprotein and of its downstream targets TCF1, cyclin D1, Axin2 and MMP7 mRNAs. Moreover, we found that the mechanism behind the TRIM32-promoted GC progression was related to the beta-catenin signalling pathway. Collectively, these data suggest that TRIM32 promotes GC cell proliferation, migration, and invasion by activating the beta-catenin signalling pathway.
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