期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 18, 期 3, 页码 371-390出版社
WILEY
DOI: 10.1111/jcmm.12236
关键词
biomarkers; microRNA; circulating microRNAs; plasma; serum; real-time PCR; profiling
资金
- NHLBI [R01 HL067176, R01 HL102464]
- American Thoracic Society
MicroRNAs (miRNAs) have emerged as important regulators in the post-transcriptional control of gene expression. The discovery of their presence not only in tissues but also in extratissular fluids, including blood, urine and cerebro-spinal fluid, together with their changes in expression in various pathological conditions, has implicated these extracellular miRNAs as informative biomarkers of disease. However, exploiting miRNAs in this capacity requires methodological rigour. Here, we report several key procedural aspects of miRNA isolation from plasma and serum, as exemplified by research in cardiovascular and pulmonary diseases. We also highlight the advantages and disadvantages of various profiling methods to determine the expression levels of plasma- and serum-derived miRNAs. Attention to such methodological details is critical, as circulating miRNAs become diagnostic tools for various human diseases.
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