期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 16, 期 9, 页码 2208-2218出版社
WILEY
DOI: 10.1111/j.1582-4934.2012.01531.x
关键词
amniotic membrane; amnion-derived cells; placenta; mesenchymal stem; stromal cells; cancer cell lines; cell proliferation
资金
- Fondazione Cariplo (Progetto Nobel)
- Ministero dell'Istruzione, dell'Universita e della Ricerca [FIRB RBNE06JBCW_002]
- Fondazione Poliambulanza
Cells derived from the amniotic foetal membrane of human term placenta have drawn particular attention mainly for their plasticity and immunological properties, which render them interesting for stem-cell research and cell-based therapeutic applications. In particular, we have previously demonstrated that amniotic mesenchymal tissue cells (AMTC) inhibit lymphocyte proliferation in vitro and suppress the generation and maturation of monocyte-derived dendritic cells. Here, we show that AMTC also significantly reduce the proliferation of cancer cell lines of haematopoietic and non-haematopoietic origin, in both cellcell contact and transwell co-cultures, therefore suggesting the involvement of yet-unknown inhibitory soluble factor(s) in this cell growth restraint. Importantly, we provide evidence that the anti-proliferative effect of AMTC is associated with induction of cell cycle arrest in G0/G1 phase. Gene expression analyses demonstrate that AMTC can down-regulate cancer cells' mRNA expression of genes associated with cell cycle progression, such as cyclins (cyclin D2, cyclin E1, cyclin H) and cyclin-dependent kinase (CDK4, CDK6 and CDK2), whilst they up-regulate cell cycle negative regulator such as p15 and p21, consistent with a block in G0/G1 phase with no progression to S phase. Taken together, these findings warrant further studies to investigate the applicability of these cells for controlling cancer cell proliferation in vivo.
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