期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 13, 期 8B, 页码 2602-2612出版社
WILEY
DOI: 10.1111/j.1582-4934.2008.00476.x
关键词
fibronectin; osseointegration; coating; integrins; biomimetic; implant
资金
- NIH [R01 EB-004496]
- Arthritis Foundation
- Georgia Tech/Emory NSF ERC on the Engineering of Living Tissues [EEC-9731643]
- Medtronic Foundation
Integrin-mediated cell adhesion to biomolecules adsorbed onto biomedical devices regulates device integration and performance. Because of the central role of integrin-fibronectin (FN) interactions in osteoblastic function and bone formation, we evaluated the ability of FN-inspired biomolecular coatings to promote osteoblastic differentiation and implant osseointegration. Notably, these biomolecular coatings relied on physical adsorption of FN-based ligands onto biomedical-grade titanium as a simple, clinically translatable strategy to functionalize medical implants. Surfaces coated with a recombinant fragment of FN spanning the central cell binding domain enhanced osteoblastic differentiation and mineralization in bone marrow stromal cell cultures and increased implant osseointegration in a rat cortical bone model compared to passively adsorbed arginine-glycine-aspartic acid peptides, serum proteins and full-length FN. Differences in biological responses correlated with integrin binding specificity and signalling among surface coatings. This work validates a simple, clinically translatable, surface biofunctionalization strategy to enhance biomedical device integration.
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