期刊
JOURNAL OF CELL SCIENCE
卷 132, 期 4, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.219709
关键词
Immune signalling; Immune synapse; In vitro reconstitution; Model membranes; Giant unilamellar vesicles
类别
资金
- European Molecular Biology Organization (EMBO) [ALTF 636-2013]
- Marie Sklodowska-Curie Intra-European Fellowships [MEMBRANE DYNAMICS-627088]
- Newton-Katip Celebi Institutional Links [352333122]
- Wolfson Foundation [18272]
- Medical Research Council [MC_UU_12010, G0902418, MC_UU_12025]
- MRC/BBSRC/ESPRC [MR/K01577X/1]
- Wellcome Trust [104924/14/Z/14, 100262Z/12/Z, 098274/Z/12/Z]
- Deutsche Forschungsgemeinschaft [1905]
- internal University of Oxford
- Wellcome Trust Sir Henry Wellcome Fellowship [107375/Z/15/Z]
- Wellcome Trust [107375/Z/15/Z, 098274/Z/12/Z] Funding Source: Wellcome Trust
- MRC [MR/M003051/1, MC_UU_00008/9, MC_UU_00008/4, 1829089, MC_UU_12010/9, MC_UU_12010/4] Funding Source: UKRI
The spatiotemporal regulation of signalling proteins at the contacts formed between immune cells and their targets determines how and when immune responses begin and end. Therapeutic control of immune responses therefore relies on thorough elucidation of the molecular processes occurring at these interfaces. However, the detailed investigation of each component's contribution to the formation and regulation of the contact is hampered by the complexities of cell composition and architecture. Moreover, the transient nature of these interactions creates additional challenges, especially in the use of advanced imaging technology. One approach that circumvents these problems is to establish in vitro systems that faithfully mimic immune cell interactions, but allow complexity to be 'dialled-in' as needed. Here, we present an in vitro systemthatmakes use of synthetic vesicles that mimic important aspects of immune cell surfaces. Using this system, we began to explore the spatial distribution of signalling molecules (receptors, kinases and phosphatases) and how this changes during the initiation of signalling. The GUV/cell system presented here is expected to be widely applicable.
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