期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 21, 页码 4774-4787出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.159038
关键词
Microtubule motor; Dynein; Cilia; Intraflagellar; transport
类别
资金
- UK Medical Research Council [G1000383-1/1, MR/K018019/1]
- University of Bristol
- MRC [MR/K018019/1, G0801848] Funding Source: UKRI
- Medical Research Council [MR/K018019/1, G0801848] Funding Source: researchfish
Cytoplasmic dynein-2 is the motor for retrograde intraflagellar transport (IFT), and mutations in dynein-2 are known to cause skeletal ciliopathies. Here, we define for the first time the composition of the human cytoplasmic dynein-2 complex. We show that the proteins encoded by the ciliopathy genes WDR34 and WDR60 are bona fide dynein-2 intermediate chains and are both required for dynein-2 function. In addition, we identify TCTEX1D2 as a unique dynein-2 light chain that is itself required for cilia function. We define several subunits common to both dynein-1 and dynein-2, including TCTEX-1 (also known as DYNLT1) and TCTEX-3 (also known as DYNLT3), roadblock-1 (also known as DYNLRB1) and roadblock-2 (also known as DYNLRB2), and LC8-1 and LC8-2 light chains (DYNLL1 and DYNLL2, respectively). We also find that NudCD3 associates with dynein-2 as it does with dynein-1. By contrast, the common dynein-1 regulators dynactin, LIS1 (also known as PAFAH1B1) and BICD2 are not found in association with dynein-2. These data explain why mutations in either WDR34 or WDR60 cause disease, as well as identifying TCTEX1D2 as a candidate ciliopathy gene.
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