期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 6, 页码 1242-1253出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.138719
关键词
Ribosome biogenesis; Pre-40S maturation; Protein kinase; CK1 delta; CK1 epsilon
类别
资金
- Swiss National Science Foundation [31003A_144221]
- Swiss National Science Foundation (SNF) [31003A_144221] Funding Source: Swiss National Science Foundation (SNF)
Biogenesis of 40S pre-ribosomal subunits requires many transacting factors, among them several protein kinases. In this study, we show that the human casein kinase 1 (CK1) isoforms delta and e are required for cytoplasmic maturation steps of 40S subunit precursors. We show that both CK1 delta and CK1 epsilon isoforms are components of pre-40S subunits, on which they phosphorylate the ribosome biogenesis factors ENP1/BYSL and LTV1. Inhibition or co-depletion of CK1 delta and CK1 epsilon results in failure to recycle a series of trans-acting factors including ENP1/BYSL, LTV1, RRP12, DIM2/PNO1, RIO2 and NOB1 from pre-40S particles after nuclear export. Furthermore, co-depletion of CK1 delta and CK1 epsilon leads to defects in 18S-E pre-rRNA processing. Together, these data demonstrate that CK1 delta and CK1 epsilon play a decisive role in triggering late steps of pre40S maturation that are required for acquisition of functionality of 40S ribosomal subunits in protein translation.
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