期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 16, 页码 3521-3534出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.149476
关键词
Acetylated tubulin; CTTNBP2; Dendritic arborization; Microtubule bundling
类别
资金
- Academia Sinica [AS-100TP- B09, AS-103-TP-B05]
- National Science Council of Taiwan [NSC 102-2321-B-001-054, NSC 102-2321-B-001-029]
Neurons are characterized by subcellular compartments, such as axons, dendrites and synapses, that have highly specialized morphologies and biochemical specificities. Cortactin-binding protein 2 (CTTNBP2), a neuron-specific F-actin regulator, has been shown to play a role in the regulation of dendritic spine formation and their maintenance. Here, we show that, in addition to F-actin, CTTNBP2 also associates with microtubules before mature dendritic spines form. This association of CTTNBP2 and microtubules induced the formation of microtubule bundles. Although the middle (Mid) region of CTTNBP2 was sufficient for its association with microtubules, for microtubule bundling, the N-terminal region containing the coiled-coil motifs (NCC), which mediates the dimerization or oligomerization of CTTNBP2, was also required. Our study indicates that CTTNBP2 proteins form a dimer or oligomer and brings multiple microtubule filaments together to form bundles. In cultured hippocampal neurons, knockdown of CTTNBP2 or expression of the Mid or NCC domain alone reduced the acetylation levels of microtubules and impaired dendritic arborization. This study suggests that CTTNBP2 influences both the F-actin and microtubule cytoskeletons and regulates dendritic spine formation and dendritic arborization.
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