期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 16, 页码 3535-3545出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.149930
关键词
Na+/H+ exchanger; Na+ absorption; Endocytosis; Trafficking; Myosin VI; Brush border
类别
资金
- National Institute of Health
- National Institute of Diabetes and Digestive and Kidney Diseases [R01-DK26523, R01-DK61765, P01-DK072084, P30-DK89502]
- Hopkins Digestive Diseases Basic and Translational Research Core Center
- Hopkins Center for Epithelial Disorders
The intestinal brush border Na+/H+ exchanger NHE3 is tightly regulated through changes in its endocytosis and exocytosis. Myosin VI, a minus-end-directed actin motor, has been implicated in endocytosis at the inter-microvillar cleft and during vesicle remodeling in the terminal web. Here, we asked whether myosin VI also regulates NHE3 movement down the microvillus. The basal NHE3 activity and its surface amount, determined by fluorometry of the ratiometric pH indicator BCECF and biotinylation assays, respectively, were increased in myosin-VI-knockdown (KD) Caco-2/Bbe cells. Carbachol (CCH) and forskolin (FSK) stimulated NHE3 endocytosis in control but not in myosin VI KD cells. Importantly, immunoelectron microscopy results showed that NHE3 was preferentially localized in the basal half of control microvilli but in the distal half in myosin VI KD cells. Treatment with dynasore duplicated some aspects of myosin VI KD: it increased basal surface NHE3 activity and prevented FSK-induced NHE3 endocytosis. However, NHE3 had an intermediate distribution along the microvillus (between that in myosin VI KD and untreated cells) in dynasore-treated cells. We conclude that myosin VI is required for basal and stimulated endocytosis of NHE3 in intestinal cells, and suggest that myosin VI also moves NHE3 down the microvillus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据