期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 12, 页码 2639-2646出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.135517
关键词
Fission yeast; Mating; Meiosis; Proteasome; TOR kinase; Mei2
类别
资金
- Hayashi Memorial Foundation for Female Natural Scientists
- Japan Society for the Promotion of Science [23570223, 21227007]
- Grants-in-Aid for Scientific Research [23570223] Funding Source: KAKEN
Target of rapamycin (TOR) kinase regulates cell metabolism and growth, acting as a subunit of two multi-protein complexes, TORC1 and TORC2. Known TORC substrates are either kinases or general factors involved in growth control. Here, we show that fission yeast TORC1, which promotes vegetative growth and suppresses sexual development, can phosphorylate Mei2 (a specific factor involved in switching the cell fate) in vitro. Alanine substitutions at the nine Mei2 phosphorylation sites stabilize the protein and promote mating and meiosis in vivo. We found that Mei2 is polyubiquitylated in vivo in a TORC1-dependent manner. Based on these data, we propose that TORC1 contributes to the suppression of sexual development by phosphorylating Mei2, in addition to controlling the cellular metabolic status.
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