期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 19, 页码 4146-4158出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.146233
关键词
Rga7; Schizosaccharomyces pombe; Actomyosin ring; Septum; F-BAR; Rho GAP; Cdc15; Imp2
类别
资金
- Ministerio de Economia y Competitividad, Spain [BFU2010-15641]
- Consejo Superior de Investigaciones Cientificas (CSIC) [JAEDOC023]
F-BAR domain proteins act as linkers between the cell cortex and cytoskeleton, and are involved in membrane binding and bending. Rga7 is one of the seven F-BAR proteins present in the fission yeast Schizosaccharomyces pombe. In addition to the F-BAR domain in the N-terminal region, Rga7 possesses a Rho GTPase-activating protein (GAP) domain at its C-terminus. We show here that Rga7 is necessary to prevent fragmentation of the contracting ring and incorrect septum synthesis. Accordingly, cultures of cells lacking Rga7 contain a higher percentage of dividing cells and more frequent asymmetric or aberrant septa, which ultimately might cause cell death. The Rga7 F-BAR domain is necessary for the protein localization to the division site and to the cell tips, and also for the Rga7 roles in cytokinesis. In contrast, Rga7 GAP catalytic activity seems to be dispensable. Moreover, we demonstrate that Rga7 cooperates with the two F-BAR proteins Cdc15 and Imp2 to ensure proper cytokinesis. We have also detected association of Rga7 with Imp2, and its binding partners Fic1 and Pxl1. Taken together, our findings suggest that Rga7 forms part of a protein complex that coordinates the late stages of cytokinesis.
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