期刊
JOURNAL OF CELL SCIENCE
卷 126, 期 12, 页码 2730-2739出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.123661
关键词
Nucleolus; Transcription; PIP2; UBF; Fibrillarin
类别
资金
- Grant Agency of the Czech Republic [P305/11/2232, 204/09/H084]
- Ministry of Education, Youth and Sports of the Czech Republic [LC545, LC06063]
- CONACYT [176598]
- Institute of Molecular Genetics, Academy of Sciences of the Czech Republic [RVO68378050]
RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that phosphatidylinositol 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2. In addition, PIP2 also binds directly to the pre-rRNA processing factor fibrillarin (Fib), and co-localizes with nascent transcripts in the nucleolus. PIP2 binding to UBF and Fib modulates their binding to DNA and RNA, respectively. In conclusion, PIP2 interacts with a subset of Pol I transcription machinery, and promotes Pol I transcription.
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