期刊
JOURNAL OF CELL SCIENCE
卷 127, 期 3, 页码 686-699出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.145037
关键词
Ral; Exocyst; Par6; Neuroblast migration; Neuronal polarity
类别
资金
- Wellcome Trust Project Grant [089236/Z/09/Z]
- National Institutes of Health [GM085146]
- American Heart Association
- National Kidney Foundation post-doctoral fellowship
- American Heart Association postdoctoral fellowship
- EMBO Long-Term and Terry Fox Foundation Canadian Cancer Society fellowships
- Cancer Research UK
- Wellcome Trust [089236/Z/09/Z] Funding Source: Wellcome Trust
- Cancer Research UK [16512] Funding Source: researchfish
Cell polarization is essential for neuronal development in both the embryonic and postnatal brain. Here, using primary cultures, in vivo postnatal electroporation and conditional genetic ablation, we show that the Ras-like small GTPase RalA and its effector, the exocyst, regulate the morphology and polarized migration of neural progenitors derived from the subventricular zone, a major neurogenic niche in the postnatal brain. Active RalA promotes the direct binding between the exocyst subunit Exo84 and the PDZ domain of Par6 through a non-canonical PDZ-binding motif. Blocking the Exo84-Par6 interaction impairs polarization in postnatal neural progenitors and cultured embryonic neurons. Our results provide the first in vivo characterization of RalA function in the mammalian brain and highlight a novel molecular mechanism for cell polarization. Given that the exocyst and the Par complex are conserved in many tissues, the functional significance of their interaction and its regulation by RalA are likely to be important in a wide range of polarization events.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据