期刊
JOURNAL OF CELL SCIENCE
卷 126, 期 16, 页码 3602-3614出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.122606
关键词
Yeast; Invasive growth; Phospholipids; Polarized growth; PtdIns(4,5)P-2; PI4P5K
类别
资金
- Centre National de la Recherche Scientifique
- Fondation pour la Recherche Medicale-BNP-Paribas
- Agence Nationale de la Recherche [ANR-09-BLAN-0299-01]
- Association pour la Recherche sur le Cancer [4979]
- FEBS
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0299] Funding Source: Agence Nationale de la Recherche (ANR)
Phosphatidylinositol phosphates are important regulators of processes such as the cytoskeleton organization, membrane trafficking and gene transcription, which are all crucial for polarized cell growth. In particular, phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2] has essential roles in polarized growth as well as in cellular responses to stress. In the yeast Saccharomyces cerevisiae, the sole phosphatidylinositol-4-phosphate 5-kinase (PI4P5K) Mss4p is essential for generating plasma membrane PtdIns(4,5)P-2. Here, we show that Mss4p is required for yeast invasive growth in low-nutrient conditions. We isolated specific mss4 mutants that were defective in cell elongation, induction of the Flo11p flocculin, adhesion and cell wall integrity. We show that mss4-f12 cells have reduced plasma membrane PtdIns(4,5)P-2 levels as well as a defect in its polarized distribution, yet Mss4-f12p is catalytically active in vitro. In addition, the Mss4-f12 protein was defective in localizing to the plasma membrane. Furthermore, addition of cAMP, but not an activated MAPKKK allele, partially restored the invasive growth defect of mss4-f12 cells. Taken together, our results indicate that plasma membrane PtdIns(4,5)P-2 is crucial for yeast invasive growth and suggest that this phospholipid functions upstream of the cAMP-dependent protein kinase A signaling pathway.
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