期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 11, 页码 2721-2731出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.100685
关键词
RHO1; GTP exchange factor; Cytokinesis; ROM2; TUS1
类别
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/E011632/1]
- BBSRC Ph.D. studentship
- research grant from the Canadian Institutes of Health Research
- BBSRC [BB/E011632/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E011632/1] Funding Source: researchfish
Rho GTPases are regulated in complex spatiotemporal patterns that might be dependent, in part at least, on the multiplicity of their GTP exchange factors (GEFs). Here, we examine the extent of and basis for functional specialisation of the Rom2 and Tus1 GEFs that activate the yeast Rho1 GTPase, the orthologue of mammalian RhoA. First, we find that these GEFs selectively activate different Rho1-effector branches. Second, the synthetic genetic networks around ROM2 and TUS1 confirm very different global in vivo roles for these GEFs. Third, the GEFs are not functionally interchangeable: Tus1 cannot replace the essential role of Rom2, even when overexpressed. Fourth, we find that Rom2 and Tus1 localise differently: Rom2 to the growing bud surface and to the bud neck at cytokinesis; Tus1 only to the bud neck, but in a distinct pattern. Finally, we find that these GEFs are dependent on different protein co-factors: Rom2 function and localisation is largely dependent on Ack1, a SEL1-domain-containing protein; Tus1 function and localisation is largely dependent on the Tus1-interacting protein Ypl066w (which we name Rgl1). We have revealed a surprising level of diversity among the Rho1 GEFs that contributes another level of complexity to the spatiotemporal control of Rho1.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据