期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 1, 页码 155-165出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.090746
关键词
Actin flow; Cell migration; Focal adhesion dynamics; Traction force
类别
资金
- Bundesministerium fur Bildung und Forschung (BMBF) [0315501]
The spatially ordered formation and disassembly of focal adhesions is a basic requirement for effective cell locomotion. Because focal adhesions couple the contractile actin-myosin network to the substrate, their distribution determines the pattern of traction forces propelling the cell in a certain direction. In the present study, we quantitatively analyzed the spatial patterning of cell-substrate adhesion in migrating cells by mapping averaged focal adhesion growth dynamics to a standardized cell coordinate system. These maps revealed distinct zones of focal adhesion assembly, disassembly and stability and were strongly interrelated with corresponding actin flow and traction force patterns. Moreover, the mapping technique enables precise detection of even minute responses of adhesion dynamics upon targeted signaling perturbations. For example, the partial inhibition of vinculin phosphorylation was followed by the reduced number of newly formed adhesions, whereas growth dynamics of existing adhesions remained unaffected.
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