期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 11, 页码 2571-2580出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.090027
关键词
Correlative light and electron microscopy; Electron microscopy; Super resolution
类别
资金
- National Science Foundation CAREER Award [MCB-0845062, 0954836]
- UNM Spatiotemporal Modeling Center, National Institutes of Heath [P50GM085273]
- Division Of Physics
- Direct For Mathematical & Physical Scien [0954836] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0845062] Funding Source: National Science Foundation
A fundamental goal in biology is to determine how cellular organization is coupled to function. To achieve this goal, a better understanding of organelle composition and structure is needed. Although visualization of cellular organelles using fluorescence or electron microscopy (EM) has become a common tool for the cell biologist, recent advances are providing a clearer picture of the cell than ever before. In particular, advanced light-microscopy techniques are achieving resolutions below the diffraction limit and EM tomography provides high-resolution three-dimensional (3D) images of cellular structures. The ability to perform both fluorescence and electron microscopy on the same sample (correlative light and electron microscopy, CLEM) makes it possible to identify where a fluorescently labeled protein is located with respect to organelle structures visualized by EM. Here, we review the current state of the art in 3D biological imaging techniques with a focus on recent advances in electron microscopy and fluorescence super-resolution techniques.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据