期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 23, 页码 5881-5886出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.113506
关键词
Bld10; Cell biology; Centrioles; Centrosomes; Cep135; Drosophila
类别
资金
- Cancer Research UK [C5395/A10530]
- Wellcome Trust [097297/Z/11/Z]
- Wellcome Trust [097297/Z/11/Z] Funding Source: Wellcome Trust
Cep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel formation, or that the cartwheel is not essential for centriole assembly in flies. Using electron tomography and super-resolution microscopy we show that centrioles can form a cartwheel in the absence of Cep135/Bld10, but centriole width is increased and the cartwheel appears to disassemble over time. Using 3D structured illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the absence of Cep135/Bld10, although the ninefold symmetry of the centriole is maintained. Thus, in flies, Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components.
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