期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 18, 页码 4189-4195出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.106005
关键词
Atomic force microscopy; AFM; Cell adhesion; Cell surface; Cell imaging; Mechanosensing; Microscopy; Single molecule
类别
资金
- National Foundation for Scientific Research (FNRS)
- Foundation for Training in Industrial and Agricultural Research (FRIA)
- Universite catholique de Louvain (Fonds Speciaux de Recherche)
- Federal Office for Scientific, Technical and Cultural Affairs (Interuniversity Poles of Attraction Programme)
- Research Department of the Communaute francaise de Belgique (Concerted Research Action)
- National Institutes of Health [SC1 GM083756]
- Deutsche Forschungsgemeinschaft [SFB944]
Living cells use cell surface proteins, such as mechanosensors, to constantly sense and respond to their environment. However, the way in which these proteins respond to mechanical stimuli and assemble into large complexes remains poorly understood at the molecular level. In the past years, atomic force microscopy (AFM) has revolutionized the way in which biologists analyze cell surface proteins to molecular resolution. In this Commentary, we discuss how the powerful set of advanced AFM techniques (e. g. live-cell imaging and single-molecule manipulation) can be integrated with the modern tools of molecular genetics (i.e. protein design) to study the localization and molecular elasticity of individual mechanosensors on the surface of living cells. Although we emphasize recent studies on cell surface proteins from yeasts, the techniques described are applicable to surface proteins from virtually all organisms, from bacteria to human cells.
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