期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 21, 页码 5138-5150出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.108373
关键词
Cell asymmetry; Excitability; Oscillation; Spontaneous polarization
类别
资金
- MEXT [23111531]
- Grants-in-Aid for Scientific Research [22111001, 22111002] Funding Source: KAKEN
A key signaling event that is responsible for gradient sensing in eukaryotic cell chemotaxis is a phosphatidylinositol (PtdIns) lipid reaction system. The self-organization activity of this PtdIns lipid system induces an inherent polarity, even in the absence of an external chemoattractant gradient, by producing a localized PtdIns (3,4,5)-trisphosphate [PtdIns(3,4,5)P-3]-enriched domain on the membrane. Experimentally, we found that such a domain could exhibit two types of behavior: (1) it could be persistent and travel on the membrane, or (2) be stochastic and transient. Taking advantage of the simultaneous visualization of PtdIns(3,4,5)P-3 and the enzyme phosphatase and tensin homolog (PTEN), for which PtdIns(3,4,5)P-3 is a substrate, we statistically demonstrated the inter-dependence of their spatiotemporal dynamics. On the basis of this statistical analysis, we developed a theoretical model for the self-organization of PtdIns lipid signaling that can accurately reproduce both persistent and transient domain formation; these types of formations can be explained by the oscillatory and excitability properties of the system, respectively.
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