期刊
JOURNAL OF CELL SCIENCE
卷 125, 期 4, 页码 965-972出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.094409
关键词
Orc; DNA replication; Origin of replication; Gal4; Drosophila S2 cells; Histone acetylation
类别
资金
- Cancer Research UK (CRUK) [C980/A6615]
Origins of replication in higher eukaryotes appear to lack specific sequence characteristics and those mapped often appear to be spread over several kilobases. This has complicated the study of site-specific events at origins of replication in vivo. Here we show that fusion of a Gal4-binding domain to proteins of the origin of replication complex (Orc) is sufficient to direct initiation to Gal4-binding sites inserted in the Drosophila S2 cell chromosome. The activation appears to go via an authentic route, taking place only in the S phase of the cell cycle and involving the formation of a prereplication complex. We have also shown that the origin-associated acetylation of histone H4 at K12 can be directed to the region of Orc binding by the presence of Orc. We expect that this system can provide a useful tool for the study of site-specific events at origins of replication in higher eukaryotes and a means to dissect Orc-dependent and Orc-independent events at origins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据