期刊
JOURNAL OF CELL SCIENCE
卷 124, 期 24, 页码 4318-4331出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.092791
关键词
Gene expression; Transcription; Microarrays; Signalling; Cytoskeleton
类别
资金
- Department of Axel Ullrich
- Wilhelm Sander-Stiftung
- Deutsche Forschungsgemeinschaft [PO 1032/1, PO 1032/2]
Monomeric actin regulates gene expression through serum response factor (SRF) by inhibiting its transcriptional coactivator myocardin-related transcription factor (MAL/MRTF). Many affected genes encode cytoskeletal components. We have analysed the migratory effects of actin-MAL signalling and of new target genes in non-invasive highly adherent cells. Expression of active MAL impaired migration of both fibroblasts and epithelial cells, whereas dominant-negative constructs and partial knockdown of MAL/MRTF enhanced motility. Knockdown of three newly characterised G-actin-regulated MAL targets, integrin alpha 5, plakophilin 2 (Pkp2) and FHL1, enhanced cell migration. All three were upregulated by external stimulation through actin-MAL-SRF signalling, and MAL and SRF were inducibly recruited to cis-regulatory elements of the integrin a5 and Pkp2 genes. Finally, the reduced migration of epithelial cells stably expressing MAL was partially reversed by knockdown of Pkp2 and FHL1. We conclude that the actin-MAL pathway promotes adhesive gene expression, including integrin a5, Pkp2 and FHL1, and that this is anti-motile for non-invasive cells harbouring high basal activity.
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