期刊
JOURNAL OF CELL SCIENCE
卷 124, 期 19, 页码 3223-3234出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.074575
关键词
Phospholipids; Cell plate; Vesicle trafficking; Endocytosis; Root development
类别
资金
- Region Ile de France and Conseil General des Yvelines
- Electron Microscopy facilities and Cell biology unit of the Imagif platform Conseil General de l'Essonne
- Bourse Canceropole Ile de France
- ANR programe blanc SphingopolaR [07-BLAN-202]
- BBSRC (UK)
- BBSRC [BBS/E/C/00004950] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00004950] Funding Source: researchfish
Acyl chain length is thought to be crucial for biophysical properties of the membrane, in particular during cell division, when active vesicular fusion is necessary. In higher plants, the process of cytokinesis is unique, because the separation of the two daughter cells is carried out by de novo vesicular fusion to generate a laterally expanding cell plate. In Arabidopsis thaliana, very-long-chain fatty acid (VLCFA) depletion caused by a mutation in the microsomal elongase gene PASTICCINO2 (PAS2) or by application of the selective elongase inhibitor flufenacet altered cytokinesis. Cell plate expansion was delayed and the formation of the endomembrane tubular network altered. These defects were associated with specific aggregation of the cell plate markers YFP-Rab-A2a and KNOLLE during cytokinesis. Changes in levels of VLCFA also resulted in modification of endocytosis and sensitivity to brefeldin A. Finally, the cytokinesis impairment in pas2 cells was associated with reduced levels of very long fatty acyl chains in phospholipids. Together, our findings demonstrate that VLCFA-containing lipids are essential for endomembrane dynamics during cytokinesis.
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