期刊
JOURNAL OF CELL SCIENCE
卷 124, 期 13, 页码 2143-2152出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.080762
关键词
Ca2+; Metabolism; Mitochondria; Mitofusin 2 (Mfn2); Unfolded protein response (UPR); Endoplasmic reticulum stress
类别
资金
- CONICYT, Chile [1080436, 15010006]
- Parkinson's UK [G-0905]
- National Institutes of Health
- American Heart Association
- American Heart Association-Jon Holden DeHaan Foundation
- NSERC [386757-2010]
- CCSRI [2010-700306]
- AI-HS [200500396]
- Ministerio de Educacion y Ciencia (MEC) [SAF2008-03803]
- Generalitat de Catalunya [2009SGR915]
- CIBERDEM (Instituto de Salud Carlos III)
- FONDAP [3110114]
- Parkinson's UK [G-0905] Funding Source: researchfish
Increasing evidence indicates that endoplasmic reticulum (ER) stress activates the adaptive unfolded protein response (UPR), but that beyond a certain degree of ER damage, this response triggers apoptotic pathways. The general mechanisms of the UPR and its apoptotic pathways are well characterized. However, the metabolic events that occur during the adaptive phase of ER stress, before the cell death response, remain unknown. Here, we show that, during the onset of ER stress, the reticular and mitochondrial networks are redistributed towards the perinuclear area and their points of connection are increased in a microtubule-dependent fashion. A localized increase in mitochondrial transmembrane potential is observed only in redistributed mitochondria, whereas mitochondria that remain in other subcellular zones display no significant changes. Spatial re-organization of these organelles correlates with an increase in ATP levels, oxygen consumption, reductive power and increased mitochondrial Ca2+ uptake. Accordingly, uncoupling of the organelles or blocking Ca2+ transfer impaired the metabolic response, rendering cells more vulnerable to ER stress. Overall, these data indicate that ER stress induces an early increase in mitochondrial metabolism that depends crucially upon organelle coupling and Ca2+ transfer, which, by enhancing cellular bioenergetics, establishes the metabolic basis for the adaptation to this response.
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