期刊
JOURNAL OF CELL SCIENCE
卷 124, 期 3, 页码 299-310出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.072728
关键词
Intravital imaging; Intravital microscopy; Cancer; Invasion; Metastasis
类别
资金
- Dutch Organization of Scientific Research (NWO) [91710330, 91796318, 175.010.2007.00]
- Dutch Cancer Society [KWF: HUBR 2009-4621]
- NIAID, NIH
Metastasis, the process by which cells spread from the primary tumor to a distant site to form secondary tumors, is still not fully understood. Although histological techniques have provided important information, they give only a static image and thus compromise interpretation of this dynamic process. New advances in intravital microscopy (IVM), such as two-photon microscopy, imaging chambers, and multicolor and fluorescent resonance energy transfer imaging, have recently been used to visualize the behavior of single metastasizing cells at subcellular resolution over several days, yielding new and unexpected insights into this process. For example, IVM studies showed that tumor cells can switch between multiple invasion strategies in response to various densities of extracellular matrix. Moreover, other IVM studies showed that tumor cell migration and blood entry take place not only at the invasive front, but also within the tumor mass at tumor-associated vessels that lack an intact basement membrane. In this Commentary, we will give an overview of the recent advances in high-resolution IVM techniques and discuss some of the latest insights in the metastasis field obtained with IVM.
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