期刊
JOURNAL OF CELL SCIENCE
卷 124, 期 13, 页码 2165-2174出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.080598
关键词
Innate immunity; Vesicular traffic; 14-3-3; Antimicrobial peptide; Drosomycin; Secretion; Drosophila
类别
资金
- NICHD
The secretion of anti-microbial peptides is recognised as an essential step in innate immunity, but there is limited knowledge of the molecular mechanism controlling the release of these effectors from immune response cells. Here, we report that Drosophila 14-3-3 epsilon. mutants exhibit reduced survival when infected with either Gram-positive or Gram-negative bacteria, indicating a functional role for 14-3-3 epsilon in innate immunity. In 14-3-3 epsilon mutants, there was a reduced release of the anti-microbial peptide Drosomycin into the haemolymph, which correlated with an accumulation of Drosomycin-containing vesicles near the plasma membrane of cells isolated from immune response tissues. Drosomycin appeared to be delivered towards the plasma membrane in Rab4- and Rab11-positive vesicles and smaller Rab11-positive vesicles. RNAi silencing of Rab11 and Rab4 significantly blocked the anterograde delivery of Drosomycin from the perinuclear region to the plasma membrane. However, in 14-3-3 epsilon mutants there was an accumulation of small Rab11-positive vesicles near the plasma membrane. This vesicular phenotype was similar to that observed in response to the depletion of the vesicular Syntaxin protein Syx1a. In wild-type Drosophila immune tissue, 14-3-3 epsilon was detected adjacent to Rab11, and partially overlapping with Syx1a, on vesicles near the plasma membrane. We conclude that 14-3-3 epsilon is required for Rab11-positive vesicle function, which in turn enables antimicrobial peptide secretion during an innate immune response.
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