期刊
JOURNAL OF CELL SCIENCE
卷 124, 期 6, 页码 873-878出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.077396
关键词
Cdk1; Cin8; Kinesin-5; Microtubules; Mitosis
类别
资金
- ISF [1043/09]
- BSF [2003141]
- Wellcome Trust [079014/Z/06/Z]
- Estonian Science Foundation [ETF 6766]
- Wellcome Trust [079014/Z/06/Z] Funding Source: Wellcome Trust
The kinesin-5 Saccharomyces cerevisiae homologue Cin8 is shown here to be differentially phosphorylated during late anaphase at Cdk1-specific sites located in its motor domain. Wild-type Cin8 binds to the early-anaphase spindles and detaches from the spindles at late anaphase, whereas the phosphorylation-deficient Cin8-3A mutant protein remains attached to a larger region of the spindle and spindle poles for prolonged periods. This localization of Cin8-3A causes faster spindle elongation and longer anaphase spindles, which have aberrant morphology. By contrast, the phospho-mimic Cin8-3D mutant exhibits reduced binding to the spindles. In the absence of the kinesin-5 homologue Kip1, cells expressing Cin8-3D exhibit spindle assembly defects and are not viable at 37 degrees C as a result of spindle collapse. We propose that dephosphorylation of Cin8 promotes its binding to the spindle microtubules before the onset of anaphase. In mid to late anaphase, phosphorylation of Cin8 causes its detachment from the spindles, which reduces the spindle elongation rate and aids in maintaining spindle morphology.
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