期刊
JOURNAL OF CELL SCIENCE
卷 123, 期 5, 页码 806-813出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.058255
关键词
PICH; Topoisomerase-II alpha; Cohesin; Spindle-assembly checkpoint; NoCut
类别
资金
- Taiwan Merit Scholarships program [NSC-095-SAF-I-564-627-TMS]
- Max-Planck Society
- Deutsche Forschungsgemeinschaft [SFB646]
- Deutsche Krebshilfe
Sister chromatid cohesion is mediated by DNA catenation and proteinaceous cohesin complexes. The recent visualization of PICH (Plk1-interacting checkpoint helicase)-coated DNA threads in anaphase cells raises new questions as to the role of DNA catenation and its regulation in time and space. In the present study we show that persistent DNA catenation induced by inhibition of Topoisomerase-II alpha can contribute to sister chromatid cohesion in the absence of cohesin complexes and that resolution of catenation is essential for abscission. Furthermore, we use an in vitro chromatid separation assay to investigate the temporal and functional relationship between cohesin removal and Topoisomerase-II alpha-mediated decatenation. Our data suggest that centromere decatenation can occur only after separase activation and cohesin removal, providing a plausible explanation for the persistence of centromere threads after anaphase onset.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据