期刊
JOURNAL OF CELL SCIENCE
卷 123, 期 21, 页码 3808-3816出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.064279
关键词
Angiogenesis; Endothelial cell; Blood vascular; Lymphatic; Prox-1; LYVE-1; Pericyte
类别
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Big C, Norfolk Fundraisers
- EU [LSHC-CT-2003-503297]
- British Heart Foundation [PG/06/071/21115T]
- Victorian Breast Cancer Research Consortium, Australia
- MRC [G0802553] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [C20059] Funding Source: researchfish
- Medical Research Council [G0802553] Funding Source: researchfish
Blood vascular cells and lymphatic endothelial cells (BECs and LECs, respectively) form two separate vascular systems and are functionally distinct cell types or lineages with characteristic gene expression profiles. Interconversion between these cell types has not been reported. Here, we show that in conventional in vitro angiogenesis assays, human BECs of fetal or adult origin show altered gene expression that is indicative of transition to a lymphatic-like phenotype. This change occurs in BECs undergoing tubulogenesis in fibrin, collagen or Matrigel assays, but is independent of tube formation per se, because it is not inhibited by a metalloproteinase inhibitor that blocks tubulogenesis. It is also reversible, since cells removed from 3D tubules revert to a BEC expression profile upon monolayer culture. Induction of the lymphatic-like phenotype is partially inhibited by co-culture of HUVECs with perivascular cells. These data reveal an unexpected plasticity in endothelial phenotype, which is regulated by contact with the ECM environment and/or cues from supporting cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据