期刊
JOURNAL OF CELL SCIENCE
卷 123, 期 7, 页码 1099-1107出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.059469
关键词
FERM domain; Epithelial integrity; Scaffold protein; EBP50/NHERF1; Apical plasma membrane
类别
资金
- GenHomme Network [02490-6088]
- Institut Curie
- National Institutes of Health [NSOS34783]
- Canadian Breast Cancer Foundation
Organization of the plasma membrane in polarized epithelial cells is accomplished by the specific localization of transmembrane or membrane-associated proteins, which are often linked to cytoplasmic protein complexes, including the actin cytoskeleton. In this study, we identified Sip1 as a Drosophila orthologue of the ezrin-radixin-moesin (ERM) binding protein 50 (EBP50; also known as the Na+/H+ exchanger regulatory factor NHERF1). In mammals, EBP50/NHERF1 is a scaffold protein required for the regulation of several transmembrane receptors and downstream signal transduction activity. In Drosophila, loss of Sip1 leads to a reduction in Slik kinase protein abundance, loss of Moesin phosphorylation and changes in epithelial structure, including mislocalization of E-cadherin and F-actin. Consistent with these findings, Moesin and Sip1 act synergistically in genetic-interaction experiments, and Sip1 protein abundance is dependent on Moesin. Co-immunoprecipitation experiments indicate that Sip1 forms a complex with both Moesin and Slik. Taken together, these data suggest that Sip1 promotes Slik-dependent phosphorylation of Moesin, and suggests a mechanism for the regulation of Moesin activity within the cell to maintain epithelial integrity.
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