期刊
JOURNAL OF CELL SCIENCE
卷 123, 期 15, 页码 2663-2671出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.064097
关键词
Basal transcription factors; Gene expression; FRAP; TFIID; TBP
类别
资金
- European community [LSHG-CT-2004-502950, HPRN-CT 00504228, LSHG-CT-2007-037445]
- Netherlands Organization for Scientific research [ALW 855.01.077/03-DYNA-F-03, CW 700.57.302]
- Netherlands Proteomics Center, ANR [05-BLAN-0396-01]
- INCA
- AICR [09-0258]
- Human Frontier Science Program [LT-0860/2005]
Gene transcription in mammalian cells is a dynamic process involving regulated assembly of transcription complexes on chromatin in which the TATA-binding protein (TBP) plays a central role. Here, we investigate the dynamic behaviour of TBP by a combination of fluorescence recovery after photobleaching (FRAP) and biochemical assays using human cell lines of different origin. The majority of nucleoplasmic TBP and other TFIID subunits associate with chromatin in a highly dynamic manner. TBP dynamics are regulated by the joint action of the SNF2-related BTAF1 protein and the NC2 complex. Strikingly, both BTAF1 and NC2 predominantly affect TBP dissociation rates, leaving the association rate unchanged. Chromatin immunoprecipitation shows that BTAF1 negatively regulates TBP and NC2 binding to active promoters. Our results support a model for a BTAF1-mediated release of TBP-NC2 complexes from chromatin.
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